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KMID : 0903619840250020129
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Journal of the Korean Society for Horticultural Science
1984 Volume.25 No. 2 p.129 ~ p.135
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Studies on Cymbidium kanran Native to Korea
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이병기/Lee, Byung Ki
곽병화/이종석/정재동/Kwack, Beyoung Hwa/Lee, Jong Suk/Chung, Jae Dong
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Abstract
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This work was carried out in order to investigate multiplepropagation devices of Cymbidium kanran native to Mt. Halla in Chejudo, Korea. Wild rhizomes collected from the habitat and aseptic rhizomes proliferated from seed germination were used for the experiment on the rhizome propagation, the shoot and root formation from the rhizomes in vitro.
Rhizome growth of Cymbidium kanran obtained from the natural habitat and the cultured on agar medium in vitro, was promoted by BA 10ppm application in the light conditions, and GA 100ppm in the dark. Shoot and root formation from the rhizomes were accelerated in BA 0.5 or 10ppm treatment in the light condition.
In rhizomes proliferated from seed germination in vitro, shoot was induced in BA 5ppm and 10ppm or BA 10ppm mixed with NAA 0.1ppm application added to the Knudson´s C medium, but it was not so in the dark. It seemed to suggest that light and cytokinin act as essential factors for
shoot formation in the rhizome culture.
Maximum growth of new rhizomes and proliferating rhizome tip of Cymbidium kanran was attained by adding NAA 0.1ppm to the basal culture medium. In combinations with the two growth regulators, the rhizome growing effect of NAA and the shoot stimulating effect of BA were shown to counteract each other in the action.
For the rhizome culture Murashige & Skoog mediyn was regarded better than Kyoto solution II, Knudson´s C or White´s medium.
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